AgrobacTransV1, Main, Exploration, bibRecord, 000003

WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots.

Identifieur interne : 000003 ( Main/Exploration ); précédent : 000002; suivant : 000004

WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots.

Auteurs : Yi Wang [République populaire de Chine] ; Chen Wang [République populaire de Chine] ; Mamy Jayne Nelly Rajaofera [République populaire de Chine] ; Li Zhu [République populaire de Chine] ; Wenbo Liu [République populaire de Chine] ; Fucong Zheng [République populaire de Chine] ; Weiguo Miao [République populaire de Chine]

Source :

PloS one [ 1932-6203 ] ; 2020.

RBID : pubmed:32479550

Descripteurs français

KwdFr :
- Champignons (génétique), Champignons (pathogénicité), Feuilles de plante (microbiologie), Génie génétique (méthodes), Génome fongique (MeSH), Hevea (génétique), Hevea (microbiologie), Interactions hôte-pathogène (génétique), Magnoliopsida (génétique), Magnoliopsida (microbiologie), Maladies des plantes (microbiologie), Maladies des plantes (prévention et contrôle), Oryza (génétique), Oryza (microbiologie), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Tabac (génétique), Tabac (microbiologie), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH), Zea mays (génétique), Zea mays (microbiologie).
MESH :
- génétique : Champignons, Hevea, Interactions hôte-pathogène, Magnoliopsida, Oryza, Tabac, Zea mays.
- microbiologie : Feuilles de plante, Hevea, Magnoliopsida, Maladies des plantes, Oryza, Tabac, Zea mays.
- méthodes : Génie génétique.
- pathogénicité : Champignons.
- prévention et contrôle : Maladies des plantes.
- Génome fongique, Régions promotrices (génétique), Régulation de l'expression des gènes fongiques, Transformation génétique, Végétaux génétiquement modifiés.

English descriptors

KwdEn :
- Fungi (genetics), Fungi (pathogenicity), Gene Expression Regulation, Fungal (MeSH), Genetic Engineering (methods), Genome, Fungal (MeSH), Hevea (genetics), Hevea (microbiology), Host-Pathogen Interactions (genetics), Magnoliopsida (genetics), Magnoliopsida (microbiology), Oryza (genetics), Oryza (microbiology), Plant Diseases (microbiology), Plant Diseases (prevention & control), Plant Leaves (microbiology), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (MeSH), Tobacco (genetics), Tobacco (microbiology), Transformation, Genetic (MeSH), Zea mays (genetics), Zea mays (microbiology).
MESH :
- genetics : Fungi, Hevea, Host-Pathogen Interactions, Magnoliopsida, Oryza, Tobacco, Zea mays.
- methods : Genetic Engineering.
- microbiology : Hevea, Magnoliopsida, Oryza, Plant Diseases, Plant Leaves, Tobacco, Zea mays.
- pathogenicity : Fungi.
- prevention & control : Plant Diseases.
- Gene Expression Regulation, Fungal, Genome, Fungal, Plants, Genetically Modified, Promoter Regions, Genetic, Transformation, Genetic.

Abstract

Promoters are very important for transcriptional regulation and gene expression, and have become invaluable tools for genetic engineering. Owing to the characteristics of obligate biotrophs, molecular research into obligate biotrophic fungi is seriously lagging behind, and very few of their endogenous promoters have been developed. In this study, a WY7 fragment was predicted in the genome of Oidium heveae Steinmann using PromoterScan. Its promoter function was verified with transient transformations (Agrobacterium tumefaciens-mediated transformation, ATMT) in Nicotiana tabacum cv. Xanthi nc. The analysis of the transcription range showed that WY7 could regulate GUS expression in both monocots (Zea mays Linn and Oryza sativa L. spp. Japonica cv. Nipponbare) and dicots (N. tabacum and Hylocereus undulates Britt). The results of the quantitative detection showed that the GUS transient expression levels when regulated by WY7 was more than 11.7 times that of the CaMV 35S promoter in dicots (N. tabacum) and 5.13 times that of the ACT1 promoter in monocots (O. sativa). GUS staining was not detected in the T1 generation of the WY7-GUS transgenic N. tabacum. This showed that WY7 is an inducible promoter. The cis elements of WY7 were predicted using PlantCARE, and further experiments indicated that WY7 was a low temperature- and salt-inducible promoter. Soluble proteins produced by WY7-hpa1Xoo transgenic tobacco elicited hypersensitive responses (HR) in N. tabacum leaves. N. tabacum transformed with pBI121-WY7-hpa1Xoo exhibited enhanced resistance to the tobacco mosaic virus (TMV). The WY7 promoter has a lot of potential as a tool for plant genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms of O. heveae.

DOI: 10.1371/journal.pone.0233911
PubMed: 32479550
PubMed Central: PMC7263610

Affiliations:

République populaire de Chine

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to stream Main, to step Corpus: 000037
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Le document en format XML

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<country xml:lang="fr">République populaire de Chine</country>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Plant Protection, Hainan University, Haikou</wicri:regionArea>
<wicri:noRegion>Haikou</wicri:noRegion>
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<author><name sortKey="Zheng, Fucong" sort="Zheng, Fucong" uniqKey="Zheng F" first="Fucong" last="Zheng">Fucong Zheng</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Hainan University, Haikou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
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<author><name sortKey="Miao, Weiguo" sort="Miao, Weiguo" uniqKey="Miao W" first="Weiguo" last="Miao">Weiguo Miao</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<wicri:noRegion>Haikou</wicri:noRegion>
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<country xml:lang="fr">République populaire de Chine</country>
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<term>Fungi (pathogenicity)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Genetic Engineering (methods)</term>
<term>Genome, Fungal (MeSH)</term>
<term>Hevea (genetics)</term>
<term>Hevea (microbiology)</term>
<term>Host-Pathogen Interactions (genetics)</term>
<term>Magnoliopsida (genetics)</term>
<term>Magnoliopsida (microbiology)</term>
<term>Oryza (genetics)</term>
<term>Oryza (microbiology)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plant Diseases (prevention & control)</term>
<term>Plant Leaves (microbiology)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Promoter Regions, Genetic (MeSH)</term>
<term>Tobacco (genetics)</term>
<term>Tobacco (microbiology)</term>
<term>Transformation, Genetic (MeSH)</term>
<term>Zea mays (genetics)</term>
<term>Zea mays (microbiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Champignons (génétique)</term>
<term>Champignons (pathogénicité)</term>
<term>Feuilles de plante (microbiologie)</term>
<term>Génie génétique (méthodes)</term>
<term>Génome fongique (MeSH)</term>
<term>Hevea (génétique)</term>
<term>Hevea (microbiologie)</term>
<term>Interactions hôte-pathogène (génétique)</term>
<term>Magnoliopsida (génétique)</term>
<term>Magnoliopsida (microbiologie)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Maladies des plantes (prévention et contrôle)</term>
<term>Oryza (génétique)</term>
<term>Oryza (microbiologie)</term>
<term>Régions promotrices (génétique) (MeSH)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Tabac (génétique)</term>
<term>Tabac (microbiologie)</term>
<term>Transformation génétique (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
<term>Zea mays (génétique)</term>
<term>Zea mays (microbiologie)</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term>
<term>Hevea</term>
<term>Host-Pathogen Interactions</term>
<term>Magnoliopsida</term>
<term>Oryza</term>
<term>Tobacco</term>
<term>Zea mays</term>
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<term>Hevea</term>
<term>Interactions hôte-pathogène</term>
<term>Magnoliopsida</term>
<term>Oryza</term>
<term>Tabac</term>
<term>Zea mays</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Engineering</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term>
<term>Hevea</term>
<term>Magnoliopsida</term>
<term>Maladies des plantes</term>
<term>Oryza</term>
<term>Tabac</term>
<term>Zea mays</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hevea</term>
<term>Magnoliopsida</term>
<term>Oryza</term>
<term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Tobacco</term>
<term>Zea mays</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génie génétique</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Fungi</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Champignons</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Plant Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Maladies des plantes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term>
<term>Genome, Fungal</term>
<term>Plants, Genetically Modified</term>
<term>Promoter Regions, Genetic</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Génome fongique</term>
<term>Régions promotrices (génétique)</term>
<term>Régulation de l'expression des gènes fongiques</term>
<term>Transformation génétique</term>
<term>Végétaux génétiquement modifiés</term>
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<front><div type="abstract" xml:lang="en">Promoters are very important for transcriptional regulation and gene expression, and have become invaluable tools for genetic engineering. Owing to the characteristics of obligate biotrophs, molecular research into obligate biotrophic fungi is seriously lagging behind, and very few of their endogenous promoters have been developed. In this study, a WY7 fragment was predicted in the genome of Oidium heveae Steinmann using PromoterScan. Its promoter function was verified with transient transformations (Agrobacterium tumefaciens-mediated transformation, ATMT) in Nicotiana tabacum cv. Xanthi nc. The analysis of the transcription range showed that WY7 could regulate GUS expression in both monocots (Zea mays Linn and Oryza sativa L. spp. Japonica cv. Nipponbare) and dicots (N. tabacum and Hylocereus undulates Britt). The results of the quantitative detection showed that the GUS transient expression levels when regulated by WY7 was more than 11.7 times that of the CaMV 35S promoter in dicots (N. tabacum) and 5.13 times that of the ACT1 promoter in monocots (O. sativa). GUS staining was not detected in the T1 generation of the WY7-GUS transgenic N. tabacum. This showed that WY7 is an inducible promoter. The cis elements of WY7 were predicted using PlantCARE, and further experiments indicated that WY7 was a low temperature- and salt-inducible promoter. Soluble proteins produced by WY7-hpa1Xoo transgenic tobacco elicited hypersensitive responses (HR) in N. tabacum leaves. N. tabacum transformed with pBI121-WY7-hpa1Xoo exhibited enhanced resistance to the tobacco mosaic virus (TMV). The WY7 promoter has a lot of potential as a tool for plant genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms of O. heveae.</div>
</front>
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<DateCompleted><Year>2020</Year>
<Month>08</Month>
<Day>20</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>08</Month>
<Day>20</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>15</Volume>
<Issue>6</Issue>
<PubDate><Year>2020</Year>
</PubDate>
</JournalIssue>
<Title>PloS one</Title>
<ISOAbbreviation>PLoS One</ISOAbbreviation>
</Journal>
<ArticleTitle>WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots.</ArticleTitle>
<Pagination><MedlinePgn>e0233911</MedlinePgn>
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<Abstract><AbstractText>Promoters are very important for transcriptional regulation and gene expression, and have become invaluable tools for genetic engineering. Owing to the characteristics of obligate biotrophs, molecular research into obligate biotrophic fungi is seriously lagging behind, and very few of their endogenous promoters have been developed. In this study, a WY7 fragment was predicted in the genome of Oidium heveae Steinmann using PromoterScan. Its promoter function was verified with transient transformations (Agrobacterium tumefaciens-mediated transformation, ATMT) in Nicotiana tabacum cv. Xanthi nc. The analysis of the transcription range showed that WY7 could regulate GUS expression in both monocots (Zea mays Linn and Oryza sativa L. spp. Japonica cv. Nipponbare) and dicots (N. tabacum and Hylocereus undulates Britt). The results of the quantitative detection showed that the GUS transient expression levels when regulated by WY7 was more than 11.7 times that of the CaMV 35S promoter in dicots (N. tabacum) and 5.13 times that of the ACT1 promoter in monocots (O. sativa). GUS staining was not detected in the T1 generation of the WY7-GUS transgenic N. tabacum. This showed that WY7 is an inducible promoter. The cis elements of WY7 were predicted using PlantCARE, and further experiments indicated that WY7 was a low temperature- and salt-inducible promoter. Soluble proteins produced by WY7-hpa1Xoo transgenic tobacco elicited hypersensitive responses (HR) in N. tabacum leaves. N. tabacum transformed with pBI121-WY7-hpa1Xoo exhibited enhanced resistance to the tobacco mosaic virus (TMV). The WY7 promoter has a lot of potential as a tool for plant genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms of O. heveae.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Yi</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Plant Protection, Hainan University, Haikou, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Chen</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Plant Protection, Hainan University, Haikou, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Rajaofera</LastName>
<ForeName>Mamy Jayne Nelly</ForeName>
<Initials>MJN</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Plant Protection, Hainan University, Haikou, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Li</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Plant Protection, Hainan University, Haikou, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Wenbo</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Plant Protection, Hainan University, Haikou, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Fucong</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Plant Protection, Hainan University, Haikou, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Miao</LastName>
<ForeName>Weiguo</ForeName>
<Initials>W</Initials>
<Identifier Source="ORCID">0000-0001-8991-5511</Identifier>
<AffiliationInfo><Affiliation>Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Plant Protection, Hainan University, Haikou, China.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>06</Month>
<Day>01</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015966" MajorTopicYN="Y">Gene Expression Regulation, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016681" MajorTopicYN="N">Genome, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D028482" MajorTopicYN="N">Hevea</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019684" MajorTopicYN="N">Magnoliopsida</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<CoiStatement>The authors have declared that no competing interests exist.</CoiStatement>
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<Month>02</Month>
<Day>22</Day>
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<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
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<Day>14</Day>
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<affiliations><list><country><li>République populaire de Chine</li>
</country>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Yi" sort="Wang, Yi" uniqKey="Wang Y" first="Yi" last="Wang">Yi Wang</name>
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<name sortKey="Liu, Wenbo" sort="Liu, Wenbo" uniqKey="Liu W" first="Wenbo" last="Liu">Wenbo Liu</name>
<name sortKey="Miao, Weiguo" sort="Miao, Weiguo" uniqKey="Miao W" first="Weiguo" last="Miao">Weiguo Miao</name>
<name sortKey="Miao, Weiguo" sort="Miao, Weiguo" uniqKey="Miao W" first="Weiguo" last="Miao">Weiguo Miao</name>
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<name sortKey="Wang, Chen" sort="Wang, Chen" uniqKey="Wang C" first="Chen" last="Wang">Chen Wang</name>
<name sortKey="Wang, Chen" sort="Wang, Chen" uniqKey="Wang C" first="Chen" last="Wang">Chen Wang</name>
<name sortKey="Wang, Yi" sort="Wang, Yi" uniqKey="Wang Y" first="Yi" last="Wang">Yi Wang</name>
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<name sortKey="Zhu, Li" sort="Zhu, Li" uniqKey="Zhu L" first="Li" last="Zhu">Li Zhu</name>
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	Serveur d'exploration sur l'agrobacterium et la transgénèse
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WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots.

WY7 is a newly identified promoter from the rubber powdery mildew pathogen that regulates exogenous gene expression in both monocots and dicots.

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